Renal Ischemia-Reperfusion Model
Ischemia-Reperfusion (IR) results from blood flow through the ischemic area, also known as reperfusion injury. It is a common complication seen in ischemic organs after they are rescued from prolonged ischemia such as heart attack and stroke.
The renal artery will be occluded unilaterally or bilaterally, followed by 24 hours of reperfusion. This will result in renal ischemia-reperfusion injury.
Experimental Protocol
Histology
Figure 1. Histological staining of renal tubular cells in SHAM, VE, and DPA treated rats.
Figure 2. Renal tubular damage score in SHAM, VE, and DPA treated rats. Renal tubular damage was decreased in DPA-treated rats after renal IR. *: p<0.01, VE vs. SHAM. #: p<0.01, DPA vs. VE.
Sample Data
Figure 3. (A) Plasma KIM-1 levels were decreased in DPA-treated rats after renal IR (ischemia-reperfusion), *: p<0.01, VE vs. SHAM; #: p<0.05, DPA vs. VE. (B) Plasma Ngal-1 levels were decreased in DPA-treated rats after renal IR, *: p<0.01, VE vs. SHAM; #: p<0.05, DPA vs. VE. (C) Plasma urea levels were decreased in DPA-treated rats after renal IR, *: p<0.05, VE vs. SHAM; #: p<0.05, DPA vs. VE. (D) Plasma creatinine levels were decreased in DPA-treated rats after renal IR, *: p<0.01, VE vs. SHAM; #: p<0.01, DPA vs. VE.
